Mavlyanova Shakhnoza Zakirovna et al.
Ministry of Health of the Republic of Uzbekistan, Uzbekistan
Title: The role of the polymorphic variant of the IIe 105Val genes of the GSTP1 in the mechanism of the development of allergic skin diseases in Uzbekistan
Biography
Biography: Mavlyanova Shakhnoza Zakirovna et al.
Abstract
Aim: It is the study of polymorphism of genes of enzymes of biotransformation of xenobiotics in patients with allergic skin diseases. Material & Methods: Patients with allergic dermatoses (AlD), DNA samples of patients and healthy donors, glutathione-transferase GSTM1 (1p13.3), GSTT1 (22q11.2) and IIe 105Val genes of the GSTP1 gene were the object and subject of the study. The study included 88 patients with AlD age ranging from 5 to 67 years. Of these, 41 are women, 50 are men. The diagnosis in all patients is confirmed by the results of the clinical examination and laboratory tests. Results: Among patients with allergic dermatoses, individuals with combined functionally inferior genotypes GSTM10/0+GSTT10/0 were more common than in the group of healthy individuals (6.8% vs. 4.1%, respectively, χ2=0.5; P=0.4; OR=1.7, 95% CI 0.405-6.979). The obtained data indicate that in individuals with zero genotypes of genes of xenobiotic enzymes GSTM1 and GSTT1 there is a tendency to the risk of allergic dermatosis development. Whereas, with the combined variants of zero and functional genotypes of polymorphism of the GSTM1 and GSTT1 genes, there were no statistically significant differences between the groups studied (p>0.05). While the frequency distribution of the occurrence of alleles and genotypes of GSTP1 in the group of patients with allergodermatosis, in comparison with the control group, significant differences were found. The functionally unfavorable allele G of the GSTP1 gene was 3.4 times statistically significantly more prevalent in the studied chromosomes of allergic dermatoses than in the population sample (χ2=10.8; P<0.05; OR=3.4; 95% CI 1.6-7.4). The associations of functionally unfavorable A/G genotypes were identified (χ2=6.9, P<0.05, OR=2.6, 95% CI 1.264-5.382) and G/G (χ2=8.0; P<0.05; OR=11.2; 95% CI 1.421-88.43) with the development of allergic dermatoses. Conclusion: Genes of glutathione transferase, polymorphism IIe 105 Val of the GSTP1 gene is the most significant marker of an increased risk of allergic skin diseases in Uzbekistan.