DNA Sequencing

DNA sequencing is the process of determining actual order of nucleotide (Adenine, Guanine, Cytosine and thymine) with in a DNA molecule. This technique is used in various fields such as medical diagnosis, biotechnology, forensic biology, virology and biological systematics and anthropology.

  • Basic methods - Maxam-Gilbert sequencing, Chain-termination methods
  • Advanced methods and de novo sequencing – 1. Shotgun sequencing, 2. Bridge PCR
  • High-throughput methods - 1. Massively parallel signature sequencing (MPSS) 2. Polony sequencing 3. 454 pyrosequencing, 4. llumina (Solexa) sequencing 5. SOLiD sequencing 6. Ion Torrent semiconductor sequencing 7. DNA nanoball sequencing
  • Methods in development- 1. Tunnelling currents DNA sequencing 2. Sequencing by hybridization 3. Sequencing with mass spectrometry 4. Microfluidic Sanger sequencing 5. Microscopy-based techniques 6. RNAP sequencing 7. In vitro virus high-throughput
  • Cancer genome sequencing

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